tMfn2 gene transfer promotes vascular smooth-muscle cells apoptosis via activation of the mitochondrial cell death pathway
10.3760/cma.j.issn.0253-3758.2009.07.018
- VernacularTitle:去除穿膜区序列的线粒体融合素2基因通过线粒体凋亡路径促进大鼠血管平滑肌细胞凋亡
- Author:
Li ZHAO
1
;
Wei ZHOU
;
Si-Kun WANG
;
Hua LIAO
;
Wen-Juan ZHANG
;
Guang-Hui CHEN
;
Xiao-Mei GUO
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
Muscle,smooth,vascular;
Apoptosis;
Adenoviridae;
Mitofusin2
- From:
Chinese Journal of Cardiology
2009;37(7):639-643
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tMfn2 gene transfer on promoting the apoptosis of vascular smooth-muscle cells (VSMCs).Methods VSMCs were infected by adenovirus-mediated tMfn2 (Adv-tMfn2) or adenovirus-mediated Mfn2 (Adv-Mfn2).FACS analysis,cell death ELISA and TUNEL staining were used to investigate the role of tMfn2 and Adv-Mfn2 gene transfer on VSMCs apoptosis.Western blot was used to analyze the protein expression of p-Akt,Bcl-2,Bax and cleaved capase-9.Results FACS and ELISA results showed that tMfn2 was superior to Mfn2 in promoting VSMCs apoptosis and tMf-n2 gene transfer induced VSMCs apoptosis in a time-dependent manner(P < 0.01).TUNEL staining evidenced that there were more positive-apoptotic VSMCs in tMfn2 group than that in Mfn2 group (P < 0.01).The protein expressions of phosphorylated Akt and Bcl-2 were significantly decreased,whereas Bax and cleaved caspased-9 protein expressions were significantly upregulated in tMfn2-transfected VSMCs.Conclusions tMfn2 transfer promoted apoptosis of VSMCs in a time dependent manner via the PL3K-Akt signaling pathway and mitochondrial apeptotic pathway.
