Efficacy comparison between transplanting microenvironmental induced and non-induced bone marrow mesenchymal stem cells in ischemic rat hearts
10.3760/cma.j.issn.0253-3758.2009.08.003
- VernacularTitle:微环境预处理的骨髓间充质干细胞治疗大鼠心肌梗死
- Author:
Xiao-Hong LI
1
;
Yong-Heng FU
;
Zai-Yi LIU
;
Guang-Feng ZHANG
;
Guang-Fu DONG
;
Qiu-Xiong LIN
;
Xi-Yong YU
Author Information
1. 广东省人民医院
- Keywords:
Myocardial infarction;
Mesenchymal stem cell transplantation;
Cell differentiation
- From:
Chinese Journal of Cardiology
2009;37(8):680-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the efficacy of transplanting bone marrow mesenchymal stem cell (BMSC) or microenvironmental induced BMSC ( iBMSC) into the ischemic myocardium of rats with myocardial infarction. Methods iBMSC was defined as BMSC co-cultured with myocardial cells for 2 weeks. The stem cells or equal volume PBS were injected into ischemic border zone 1 wk after experimental infarction. Cardiac performance was evaluated at 1, 2, and 4 wk after cell transplantation by echocardiography and analyzed histologically at 4 wk after cell transplantations. Results Compared with PBS group, both BMSC and iBMSC transplantations reduced infarct size. iBMSC enhanced the beneficial effects of BMSC on improving cardiac function (FS: 28.5% ±4.3% in PBS, 29.0% ±2.0% in BMSC and 45. 1% ±3. 1% in iBMSC group at 4 weeks post transplantation, iBMSC group vs. PBS group P <0. 05, iBMSC group vs. BMSC group P <0. 05). Immunofluorescence microscopy results revealed co-localization of SPIO-labeled transplanted cells with cardiac markers for cardiomyocytes, indicating regeneration of damaged myocardium. Conclusion Our data suggest that iBMSC implantation is more effective on improving cardiac function than BMSC implantation in this model. iBMSC might serve as a new promising therapeutic cell source for regenerating ischemic myocardium in patients with post-infarction heart failure.
