Intracoronary and hypodermic injection of granulocyte colony-stimulating factor improved cardiac function in Swine with chronic myocardial ischemia
10.3760/cma.j.issn.0253-3758.2009.08.004
- VernacularTitle:冠状动脉内粒细胞集落刺激因子注射治疗猪慢性缺血性心脏病
- Author:
Rong-Chong HUANG
1
;
Kang YAO
;
Hao LU
;
Jun YANG
;
Hong-Cheng SHI
;
Yi-Qi ZHANG
;
Zhe-Yong HUANG
;
Shu-Ning ZHANG
;
Shan YANG
;
Ai-Jun SUN
;
Yun-Zeng ZOU
;
Jun-Bo GE
Author Information
1. 大连医科大学附属第一医院
- Keywords:
Myocardial ischemia;
Granulocyte colony-stimulating factor;
Ventricular function;
Apoptosis
- From:
Chinese Journal of Cardiology
2009;37(8):685-691
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To compare the efficacy and feasibility between intracoronary and hypodermic injection of granulocyte colony-stimulating factor ( G-CSF) on improving cardiac function in a Swine model of chronic myocardial ischemia. Methods Eighteen Swine underwent placement of ameriod constrictor on left circumflex coronary artery. The presence of myocardial ischemia was verified at four weeks after the operation, and the animals were then randomly assigned into three groups ( n = 6 each) : ( 1 ) administration of vehicle (control) , (2) hypodermic injection of G-CSF(5 μg · kg-1·d-1 ) for five days (IH) , and (3) intracoronay injection of a bonus G-CSF (60 μg/kg) (IC). Coronary angiogram, cardiac MRI, and 18F-FDG-SPECT/99mTc-SPECT ( DISA-SPECT ) measurements were performed at pre-administration and at 4 weeks post administration. Global heart function such as left ventricular end-diatolic volume ( LVEDV ) , left ventricular end-systolic volume ( LVSDV) and left ventricular ejection fraction (LVEF), myocardial perfusion, myocardial viability and myocardial infarct area were evaluated. Myocardial vWF, Bcl-2 and Bax expressions were detected by Western blot and RT-PCR. Results MRI data showed that left ventricular dilation and dysfunction were similarly prevented in IH and IC G-CSF treated animals at eight weeks after the operation. SPECT revealed that both IH and IC G-CSF equally improved the regional contractility of chronic myocardial ischemia and increased myocardial viability. Myocardial infarct size was also reduced after both G-CSF treatments as detected by MRI. Intracoronay injection of G-CSF did not lead to angiogenesis in other organs. G-CSF treatments were also associated with a significant reduction in myocardial apoptosis and significant increase in angiogenesis. Conclusions Both intracoronary and hypodermic injection of G-CSF were safe and feasible and could equally improve cardiac function and increase angiogenesis in this Swine model of chronic myocardial ischemia.
