Study on application of amphotericin B in the perforated whole-cell patch clamp technique.
- Author:
Liang ZHANG
;
Chong-Luo FU
- Publication Type:Journal Article
- MeSH: Amphotericin B; pharmacology; Animals; Mice; Neurons; drug effects; Patch-Clamp Techniques
- From: Chinese Journal of Applied Physiology 2014;30(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEEstablished with amphotericin B perforated patch-clamp technique, to study the electrophysiological properties of calyx synapses.
METHODSIn the present experiments, we studied the application of perforated patch clamp technique on the calyx synapses of mice with Amphotericin B.
RESULTSThe use of Amphotericin B significantly slowed down the decay of channel currents and the optimum concentration was 400 microg/ml.
CONCLUSIONThe syudy developed a stable of perforated patch clamp whole cell recording technique, could be more effecitve, more real reaction neurons electrophysiological characteristics of the channel current. Our work might provide the basic information to future users studying the signal transmission and regulation of auditory system of rodents.