Effects of long-term sleep deprivation on mitochondria stress in locus coeruleus and the tyrosine hydroxylasic projection in mice.
- Author:
Jing ZHANG
;
Jing MA
;
Guang-Fa WANG
- Publication Type:Journal Article
- MeSH: Animals; Chaperonin 60; metabolism; Locus Coeruleus; metabolism; physiology; Mice; Mitochondria; metabolism; Mitochondrial Proteins; metabolism; Oxidative Stress; physiology; Sirtuin 3; metabolism; Sleep Deprivation; Tyrosine; metabolism
- From: Chinese Journal of Applied Physiology 2014;30(2):153-156
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the changes of mitochondria stress in locus coeruleus and the tyrosine hydroxylasic projection after long-term sleep deprivation.
METHODSSleep deprivation mice model was set up by employing "novel environments" method. The expression of NAD -dependent deacetylase Sirtuin type 3 (SIRT3), which regulates mitochondrial energy production and oxidative stress, and heat shock protein 60 (HSP60), a major biomarker of mitochondrial stress, and the tyrosine hydroxylasic projection from locus coeruleus were analyzed after a 5-day sleep deprivation.
RESULTSCompared to the control group, the expression of SIRT3 in locus coeruleus was significantly decreased in respouse to long-term sleep deprivation, while the expression of HSP60 was significantly increased. In addition, relative to control group, pereentage area of the tyrosine hydroxylasic projection to anterior cingulate cortex was substantial decreased in long-term sleep deprivation group.
CONCLUSIONLong-term sleep deprivation induced the decreased level of SIRT3 expression and the elevation of mitochondrial stress in locus coenileus, which may further lead to the loss of tyrosine hydroxylasic projection in mice.