The role of arecoline on hepatic insulin resistance in type 2 diabetes rats.
- Author:
Hong-Yan LING
;
Qi-Xin YAO
;
Zhu-Qing QI
;
Si-Si YANG
;
Jian-Qin HE
;
Kai-Fang ZHANG
;
Bi HU
- Publication Type:Journal Article
- MeSH: Animals; Arecoline; pharmacology; Diabetes Mellitus, Experimental; metabolism; Diabetes Mellitus, Type 2; metabolism; Glucose Transporter Type 4; metabolism; Glucose-6-Phosphatase; metabolism; Insulin Resistance; Interleukin-6; metabolism; Intracellular Signaling Peptides and Proteins; metabolism; Liver; drug effects; metabolism; Male; Phosphoenolpyruvate Carboxykinase (GTP); metabolism; Proto-Oncogene Proteins c-akt; metabolism; Rats; Rats, Wistar; Receptors, Cytoplasmic and Nuclear; metabolism; Receptors, Steroid; metabolism; Tumor Necrosis Factor-alpha; metabolism
- From: Chinese Journal of Applied Physiology 2014;30(3):208-212
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of arecoline on hepatic insulin resistance in type 2 diabetes rats and to elucidate its possible mechanism.
METHODSForty five Wistar rats were fed with high fructose diet for 12 weeks to induce type 2 diabetic rat model. rats were randomly divided into 5 groups (n = 8): control group, model group and model group were treated with different dose (0, 0.5, 1, 5 mg/kg) of arecoline. After 4 weeks, the fasting blood glucose, blood lipid and insulin level measured , mRNA expression of liver constitutive androstane receptor (CAR), pregnane X receptor (PXR), glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) were detected by reverse transcription polymerase chain reaction (RT-PCR), the protein expression of p-AKT and glucose transporter4 (GLUT4) were detected by Western blot.
RESULTS1.5 mg/kg arecoline could significantly decrease the level of fasting blood glucose, blood lipid, blood insulin level and liver G6Pase, PEPCK, IL-6, TNF-alpha mRNA level in type 2 diabetes rats. 1.5 mg/kg arecoline also could significantly increase CAR, PXR mRNA level and p-AKT and GLUT4 protein expression.
CONCLUSIONArecoline improved hepatic insulin resistance in type 2 diabetes rats by increasing the mRNA levels of CAR and PXR leading to the creased glucose metabolism and inflammation related genes expression.