The measurement of the third-order branches of the mesenteric artery tone by microvascular ring technique.
- Author:
Hao LI
;
Su-Li ZHANG
;
Yan YANG
;
Xiao-Rong ZENG
;
Hui-Rong LIU
- Publication Type:Journal Article
- MeSH: Acetylcholine; pharmacology; Animals; In Vitro Techniques; Male; Mesenteric Arteries; drug effects; physiology; Nitroprusside; pharmacology; Norepinephrine; pharmacology; Rats; Vasoconstrictor Agents; pharmacology; Vasodilation; physiology; Vasodilator Agents; pharmacology
- From: Chinese Journal of Applied Physiology 2014;30(3):214-217
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn our study, the function of the third-order branches of the mesentenc artery was measured by microvascular ring technique, which can be used to detect microvascular function in some disease related to microvascular dysfunction.
METHODSIsolated, fixed, standardized and then activated the third-order branches of rat mesenteric artery. Microvascular tone was measured by systolic and diastolic drags respectively, with the help of DMT tension apparatus and PowerLab data acquisition system.
RESULTSThe third-order branches of rat mesenteric artery showed excellent response to vasoactive drugs. The contraction effect of norepinephrine (NE) reached 19 in mN. When acetylcholine (Ach) or sodium nitroprusside (SNP) of 10(9)-10(5)mol/L was added, vascular tones showed gradient drop: 80% of maximal relaxation when adding ACh, while 95% of maximal relaxation when adding SNP.
CONCLUSIONThe third-order branches of the mesenteric artery function was successfully detected by using microvascular ring technique.