Effect of Jinguo Weikang Capsule on proto-oncogene expression of gastric mucosa in rats with gastric precancerous lesions.
- Author:
Shu-wen SHEN
1
;
Ya YUWEN
;
Zhen-lu ZHANG
;
Sheng DONG
;
Jun-tian LIU
;
Xiao-mei WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; pharmacology; Gastric Mucosa; drug effects; metabolism; pathology; Gene Expression Regulation, Neoplastic; drug effects; Immunohistochemistry; Oncogene Proteins; metabolism; Precancerous Conditions; metabolism; pathology; Proto-Oncogene Proteins; metabolism; Proto-Oncogene Proteins c-myc; metabolism; Rats; Rats, Sprague-Dawley; Receptor, Epidermal Growth Factor; metabolism; Tumor Suppressor Protein p53; metabolism; ras Proteins; metabolism
- From: Chinese journal of integrative medicine 2008;14(3):212-216
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the effect of Jinguo Weikang Capsule [see text] on the gene expression of H-ras, epidermal growth factor receptor (EGFR), P53 and C-myc of the gastric mucosa in rats with gastric precancerous lesions, and to investigate the action mechanism of JWC on gastric precancerous lesions.
METHODSA rat model with paratypical proliferation of the gastric epithelium mucosa was established by using 60Co irradiation. Rats were divided into the normal group, model group, high-, medium-, low-dose JWC treatment groups, and the vitacoenzyme control group, and were treated for 30 days. The expression of H-ras, EGFR, P53 and C-myc genes of the gastric mucosa was detected by using immunohistochemical methods.
RESULTSThe expression and over-expression rates of H-ras, EGFR, P53 and C-myc gene in the high-and medium-dose JWC treatment groups were significantly lower (P<0.05) as compared with those of the model group.
CONCLUSIONJWC can inhibit the expression of the H-ras, EGFR, P53 and C-myc genes expression of the gastric mucosa in rats, which may be one of mechanisms involved in suppressing or reversing gastric carcinogenesis.
