Amplification methods of DNA from paraffin-embedded tissues of oral leukoplakia.
- Author:
Yuan HE
1
;
Qian-ming CHEN
;
Bing-qi LI
Author Information
- Publication Type:Journal Article
- MeSH: Ataxia Telangiectasia Mutated Proteins; Cell Cycle Proteins; genetics; DNA-Binding Proteins; genetics; Humans; Leukoplakia, Oral; genetics; Paraffin Embedding; Polymerase Chain Reaction; methods; Protein-Serine-Threonine Kinases; genetics; Tumor Suppressor Proteins; genetics
- From: Chinese Journal of Stomatology 2007;42(12):744-746
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo set up a feasible method for detection of objective genes from paraffin-embedded tissues of oral leukoplakia (OLK).
METHODSTwenty-five pieces of formalin-fixed, paraffin-embedded tissues of OSCC were selected and ATM gene was detected respectively by 3 methods: the microdissection-nested PCR method, proteinase K-PCR method and conventional phenol-chloroform-PCR method. The positivity rates were compared statistically.
RESULTSThe positivity rates of these 3 methods were 84%, 52% and 64% respectively. Significant difference was found in positivity rate between the microdissection-nested PCR method and the proteinase K-PCR method (P = 0.032).
CONCLUSIONSThe microdissection-nested PCR method merits recommendation because it is more efficient, easy to perform and has the advantage of less sample amount.
