The Role of CTGF in Osteosarcoma Progression.
10.5292/jkbjts.2014.20.1.1
- Author:
Ilkyu HAN
;
Mi Ra LEE
;
Han Soo KIM
- Publication Type:Original Article
- Keywords:
osteosarcoma;
connective tissue growth factor;
CCN;
CTGF;
cancer progression
- MeSH:
Blotting, Western;
Cell Line;
Cell Proliferation;
Connective Tissue Growth Factor;
Osteosarcoma*;
RNA, Messenger;
RNA, Small Interfering;
Transfection
- From:The Journal of the Korean Bone and Joint Tumor Society
2014;20(1):1-6
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To examine the expression of Connective Tissue Growth Factor (CTGF) in osteosarcoma and to evaluate its role in osteosarcoma invasion and proliferation. MATERIALS AND METHODS: The mRNA expression of CTGF from 23 patient-derived osteosarcoma cell lines was examined, and the role of CTGF in cell invasion and proliferation was examined using siRNA transfection. RESULTS: The over-expression of CTGF mRNA was observed in 17 cell lines (74%). CTGF-specific siRNA transfection into SaOS-2 and MG63 cell lines resulted in efficient knockdown of CTGF expression on Western blot analysis. siRNA transfected cells showed decreased migration on Matrigel invasion assay and decreased cell proliferation on WST-1 assay. CONCLUSION: These results indicated that the CTGF expression may play an important role in osteosarcoma progression, and may be a therapeutic target of osteosarcoma.
