Expression and significance of stromal cell-derived factor-1α and its receptor CXCR4 in human dental pulp cells
10.3321/j.issn:1002-0098.2008.03.006
- VernacularTitle:基质细胞衍生因子1α及其受体CXCR4在人牙髓细胞中的表达
- Author:
Qi-Mei GONG
1
;
Jun-Qi LING
;
Hong-Wei JIANG
;
Yu DU
;
Fang YANG
Author Information
1. 中山大学
- Keywords:
Receptors,CXCR4;
Cell proliferation;
Cell movement;
Stromal cell-derived factor-1
- From:
Chinese Journal of Stomatology
2008;43(3):146-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of CXCR4 in cultured human dental pulp cells (HDPC) in vitro and the corresponding ligand SDF-1α level of HDPC supernatants stimulated by lipopolysaccharide(LPS)and tumor necrosis factor-α(TNF-α),and to explore the role of SDF-1αon the proliferation and the migration of HDPC.Methods The expression of CXCR4 in HDPC was detected by immunocytochemistry technique and indirect immunofluorescence technique.The culture supernatants of HDPC were collected after HDPC had been simulated by LPS and TNF-α of difierent concentrations for 48h and then the SDF-1α level was assayed by quantitative sandwich ELISA.Meanwhile,the effects of recombinant human SDF-1α(rhSDF-1α)on the proliferation and the migration of HDPC at different concentrations were observed by MTT and Boyden Chamber Assay.Results CXCR4 was expressed in cytomembrane of HDPC and SDF-1α was secreted into their normal cell supematants with a concentration of(4513.55±962.92)ng/L. The secretion of SDF-1α was both significantly decreased by stimulation with LPS and TNF-α(P＜0.05).In addition,rhSDF-1α stimulated the HDPC proliferation at the concentrations of 50,100,200μg/L(P＜0.01)and increased the chemotactic migration of HDPC significantly after 9h's incubation with the concentrations of 50,100μg/L(P＜0.05).Conclusions SDF-1α accelerated the proliferation and the migration of HDPC which expressed CXCR4.SDF-1-CXCR4 axis may play a role in repair of pulp injury.
