Two-dimensional electrophoresis analysis of the protein expression of dental pulp in different conditions.
- Author:
Chen LIN
1
;
Min NIE
;
Lu ZHANG
;
Zhi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Dental Caries; metabolism; Dental Pulp; metabolism; Down-Regulation; Electrophoresis, Gel, Two-Dimensional; Humans; Proteome; analysis; biosynthesis; Proteomics; methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Chinese Journal of Stomatology 2008;43(3):154-156
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the different responses of dental pulp to moderate caries and thermal stimulation by proteomics.
METHODSTwo-dimensional electrophoresis(2-DE) was performed to obtain the 2-D gel electrophoresis patterns of dental pulp. Mass spectrometry (MS) was used to analyze several different selected spots in the expression proteins.
RESULTSNo significant difference in protein expression was found between normal and moderate carious dental. Two protein spots were absent in heat-damaged group and 8 spots showed significantly down-regulated. Seven proteins were identified by MS.
CONCLUSIONSIn the present study, no significant difference in the pulp protein expression was detected between the healthy and moderate carious pulp tissues. However, down-regulation of pulp protein in thermal stimulation was observed.
