Comparison of the attachment and growth characteristics between human junctional epithelium and oral epithelium cells
10.3321/j.issn:1002-0098.2008.04.018
- VernacularTitle:人牙龈结合上皮和口腔龈上皮细胞的附着生长特性比较
- Author:
De-Yi LI
1
;
Li-Zhen WANG
;
Qian JIANG
;
Xiu-Li ZHANG
;
Bin ZHANG
Author Information
1. 上海交通大学医学院附属第九人民医院
- Keywords:
Epithelial attachment;
Gingiva;
Cell culture techniques;
Cell growth processes
- From:
Chinese Journal of Stomatology
2008;43(4):240-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the attachment and growth characteristics between human junctional epithelium(JE)and oral epithelium cells.Methods The healthy JE biopsies were derived from the human teeth extracted due to impaction or orthodontic purpose.Enzyme digestion was used to isolate JE cells,which were then cultured in DKGM.The co-culture model of JE cell-tooth slice was buih up by adding 3 decalcification cementum slices(5mm×3mm×1mm)into sterilized plate containing 1ml of JE cells (5×108/L),21 slices all together,and incubated in an atmosphere containing 5% CO2 at 37℃ for 1-14days.The attachment structrure was observed under transmission electron microscope,and the OE cells was used as control.Results The human JE cells were polymorphous in shape and CKl9 posotive,while OE eells were consisted of equal and closely packed epithelial-like cells in a paving stone arrangement,and CK19 was only strained in a few cells.There were a few cells in JE-slice when co-cultured for 1-3 days.and electron dense plaques on the JE cell surface of the attached slice were observed at 9 days.and 2-3 layer of JE cells and hemidesmosome-like structure formed within 11-14 days.There were more OE cells within 1-3 days,electron dense plaques appeared at 7 days,and stratified epithelium and hemidesmosome-like structure formed in OE-slice at 9 days.Conclusions The cultured JE cells were immature and lower differentiated epithelial cells which were different from OE cells.Under the same condition the growth and attachment of JE cells on the cementum slice surface were slower than that of OE cells.Their attachment strength needs further study.
