Preliminary study on herpes simplex virus type 1 infection of human oral epithelial cell in vitro.
- Author:
Jie ZHAO
1
;
Wei-Bin SUN
;
Juan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cercopithecus aethiops; Epithelial Cells; virology; Herpesvirus 1, Human; pathogenicity; Humans; Mouth; cytology; Vero Cells
- From:Chinese Journal of Stomatology 2008;43(4):244-245
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the way of herpes simplex virus type 1 (HSV-1) infecting human oral epithelial cell in vitro.
METHODSAbundance of HSV-1 strains were used to infect human oral epithelial cells. The culture supernatant was collected to infect Vero cells again. Morphology of HSV-1 was identified by inverted microscope and transmission electron microscope. Nucleic acid of the virus was detected by PCR.
RESULTSThe infected human oral epithelial cells didn't display obvious cytopathic effect (CPE) while Vero cells infected by the culture supernatant showed typical CPE. The virus particles were observed in Vero cells. The nucleic acid of HSV-1 could be detected in infected human oral epithelial cells by PCR.
CONCLUSIONSHSV-1 could successfully infect human oral epithelial cells.
