Preliminary study on Herpes simplex virus type 1 infection of human oral epithelial cell in vitro
10.3321/j.issn:1002-0098.2008.04.019
- VernacularTitle:单纯疱疹病毒1型体外感染人口腔上皮细胞的初步探讨
- Author:
Jie ZHAO
1
;
Wei-Bin SUN
;
Juan WANG
Author Information
1. 南京医科大学口腔医学院
- Keywords:
Herpesvirus 1,human;
Epithelial cells;
Microscopy,electron,transmission
- From:
Chinese Journal of Stomatology
2008;43(4):244-245
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the way of herpes simplex virus type 1(HSV-1) infecting human oral epithelial cell in vitro.Methods Abundance of HSV-1 strains were used to infect human oral epithdial cens.The culture supernatant was collected to infect Vero cells again.Morphology of HSV-1 was identified by inverted microscope and transmission electron microscope.Nucleic acid of the virus was detected by PCR.Results The infected human oral epithelial cells didn't display obvious cytopathic effect (CPE)while Vero cells infected by the culture supernatant showed typical CPE.The virus particles were observed in Vero cells.The nucleic acid of HSV-1 could be detected in infected human oral epithelial cells by PCR.Conclusions HSV-1 could successfully infect human oral epithelial cells.
