Monocyte chemoattractant protein-1 regulations of human gingival fibroblasts by Porphyromonas gingivalis with different fimA genotypes.
- Author:
Lei ZHAO
1
;
Ya-fei WU
;
Yu-ling OUYANG
;
Jing-yi ZHANG
;
Bin CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Chemokine CCL2; metabolism; Fibroblasts; metabolism; Fimbriae Proteins; genetics; Genotype; Gingiva; cytology; microbiology; Humans; Porphyromonas gingivalis; genetics
- From:Chinese Journal of Stomatology 2008;43(5):269-272
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanism of monocyte chemoattractant protein-1 (MCP-1) regulations of human gingival fibroblasts (HGF) by challenge of Porphyromonas gingivalis (Pg) with different fimA genotypes.
METHODSPg ATCC 33277 (type I), WCSP115 (type II), WCSP1.5 (type III), W83 (type IV) were assessed for their inductions of MCP-1 expression in HGF. MCP-1 mRNA levels of HGF were determined by real-time RT-PCR and MCP-1 protein levels in culture supernatant by ELISA at different time intervals (1 h, 3h, 6h and 12h) following continuous co-culture of bacteria with HGF.
RESULTSMCP-1 mRNA and protein levels were both up-regulated when HGF co-cultured with different Pg fimA genotypes. Type II was stronger than other fimA genotypes, HGF expressed significantly great amount of MCP-1 mRNA [(25.75 +/- 3.12)-(326.69 +/- 35.35)] and protein [(178.20 +/- 46.20)-(443.46 82.19) ng/L] for different time periods; While Type III was weaker than other fimA genotypes, and the level of MCP-1 mRNA was [ (4.16 +/- 0.82)-(94.17 +/- 18.56)] and protein [(86.95 +/- 23.90)-(264.01 +/- 28.59) ng/L](P < 0.05).
CONCLUSIONSfimA genotypes of Pg are related with the inductions of MCP-1, which might indicate fimA genotype is associated with pathogenesis of Pg.
