Monocyte chemoattractant protein-1 regulations of human gingival fibroblasts by Porphyromonas gingivalis with different fimA genotypes
10.3321/j.issn:1002-0098.2008.05.004
- VernacularTitle:不同fimA基因型牙龈卟啉单胞菌刺激牙龈成纤维细胞后单核细胞趋化蛋白1的表达
- Author:
Lei ZHAO
1
;
Ya-Fei WU
;
Yu-Ling OUYANG
;
Jing-Yi ZHANG
;
Bin CHEN
Author Information
1. 四川大学华西口腔医学院
- Keywords:
Porhyromonas gingivalis;
Genotype;
Fibroblasts;
Chemokine CCL2
- From:
Chinese Journal of Stomatology
2008;43(5):269-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of monocyte chemoattractant protein-1 (MCP-1)regulations of human gingival fibroblasts(HGF)by challenge of Porphyromonas gingivalis(Pg)with different fimA genotypes.Methods Pg ATCC 33277(type Ⅰ),WCSP115(type Ⅱ),WCSP1.5(type Ⅲ),W83(type Ⅳ)were assessed for their inductions of MCP-1 expression in HGF.MCP-1 mRNA levels of HGF were determined by real-time RT-PCR and MCP-1 protein levels in culture supernatant by ELISA at different time intervals(1h,3h,6h and 12h)following continuous co-culture of bacteria with HGF.Results MCP-1 mRNA and protein levels were both up-regulated when HGF co-cultured with different Pg fimA genotypes.Type Ⅱ was stronger than other fimA genotypes,HGF expressed significantly great amount of MCP-1 mRNA[(25.75±3.12)-(326.69±35.35)]and protein[(178.20±46.20)-(443.46±82.19)ng/L]for different time periods;While Type Ⅲ was weaker than other fimA genotypes,and the level of MCP-1 mRNA was[(4.16±0.82)-(94.17±18.56)]and protein[(86.95±23.90)-(264.01±28.59)ng/L](P<0.05).Conclusions fimA genotypes of Pg are related with the inductions of MCP-1,which might indicate fimA genotype is associated with pathogenesis of Pg.
