An animal experiment for the regeneration of periodontal defect by application of the dual-release chitosan thermosensitive hydrogel system
10.3321/j.issn:1002-0098.2008.05.005
- VernacularTitle:骨形成蛋白和氯己定双缓释的壳聚糖温敏凝胶用于牙周组织再生的实验研究
- Author:
Zhi-Wei MA
1
;
Yong-Jie ZHANG
;
Rong WANG
;
Qin-Tao WANG
;
Guang-Ying DONG
;
Zhi-Fen WU
Author Information
1. 第四军医大学口腔医学院
- Keywords:
Bone morphogentic proteins;
Chitosan;
Beta-cyclodextrins;
Animal experimentation
- From:
Chinese Journal of Stomatology
2008;43(5):273-277
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of the self-made chitosan thermosensitive hydrogel system with dual-release bone morphogenetic protein and chlorhexidine on periodontal defects repair.Methods The furcation defect model was established on dog premolar.The models were divided into five groups,including three experimental groups,one control group and one blank control group.The hydrogel with the chlorhexidine/β-cyclodextrin inclusion complexes(IC)/rhBMP-2,hydrogel with rhBMP-2,hydrogel with IC,the pure hydrogel were applied to the defects of the four groups,respectively,and the blank control group did not receive any agent.The dogs were sacrificed 8 weeks later and the periodontal regeneration and gingival condition were observed by histological examination.Results Obvious periodontal tissue regeneration was found in group one and two.The heights of new bone reached 99.2% of the defects in group one,87.8%,63.6%,37.0% and 34.3% in group two,three,four and blank control groups,respectively.The inflammation of the affected gingiva showed less significant in group one and group three than in the other groups.Condusions rhBMP-2 and chlorhexidine played their independent role in repairing periodontal defects and the dual-release chitosan thermosensitive hydrogel system is effective and convenient to use.
