Protective effect of baicalin on experimental periodontitis in rats and its possible mechanisms.
- Author:
Xia CAI
1
;
Cheng-zhang LI
;
Zheng-guo CAO
;
Ge-fei DU
;
Li-hua LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Flavonoids; pharmacology; Gingiva; drug effects; metabolism; Male; Matrix Metalloproteinases; metabolism; Periodontitis; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Stomatology 2008;43(5):281-285
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of baicalin on the experimental periodontitis in rats, as well as the expression of MMP-1, MMP-2, MMP-9.
METHODSTwenty-seven adult male Sprague-Dawley rats were divided into three groups, with 9 rats in each group. A nylon thread was placed around the lower first molars of rats, which were sacrificed after 7 days. Baicalin (200 mg/kg) was administered to the experimental group by oral gavage, starting one day before the induction of periodontitis. The negative control group received vehicle (0.5% carboxymethylcellulose) alone. The blank control group did not get induction of periodontitis. The alveolar bone loss (ABL) and the area fraction (AA% ) occupied by collagen fibers were assessed. MMP-1, MMP-2 and MMP-9 protein expressions in the gingiva were detected by immunohistochemistry.
RESULTSBaicalin treatment significantly decreased ABL compared with the negative control group (P = 0.009). AA% of collagen fibers was significantly higher in baicalin-treated group than in the negative control group (P = 0.047). Baicalin treatment significantly down-regulated the protein expression for MMP-1 (P = 0.023) and MMP-9 (P = 0.042) and decreased the expression for MMP-2 (P = 0.099) compared with the negative control group.
CONCLUSIONSBaicalin protects against tissue damage in ligature-induced periodontitis in rats, which might be mediated in part by its inhibitory effect on the expression of MMP-1, MMP-2 and MMP-9.