Protective effect of baicalin on experimental periodontitis in rats and its possible mechanisms
10.3321/j.issn:1002-0098.2008.05.007
- VernacularTitle:黄芩苷对大鼠实验性牙周炎的保护效应及作用机制研究
- Author:
Xia CAI
1
;
Cheng-Zhang LI
;
Zheng-Guo CAO
;
Ge-Fei DU
;
Li-Hua LIU
Author Information
1. 浙江大学医学院附属口腔医院
- Keywords:
Periodontitis;
Models,animal;
Matrix metalloproteinases;
Baicalin
- From:
Chinese Journal of Stomatology
2008;43(5):281-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of baicalin on the experimental periodontitis in rats,as well as the expression of MMP-1,MMP-2,MMP-9.Methods Twenty-seven adult male Sprague-Dawley rats were divided into three groups,with 9 rats in each group.A nylon thread was placed around the lower first molars of rats,which were sacrificed after 7 days.Baicalin(200 mg/kg)was administered to the experimental group by oral gavage,starting one day before the induction of periodontitis.The negative control group received vehicle(0.5% carboxymethylcellulose)alone.The blank control group did not get induction of periodontitis.The alveolar bone loss(ABL)and the area fraction(AA%)occupied by collagen fibers were assessed.MMP-1,MMP-2 and MMP-9 protein expressions in the gingiva were detected by immunohistochemistry.Results Baicalin treatment significantly decreased ABL compared with the negative control group(P=0.009).AA% of collagen fibers was significantly higher in baicalin-treated group than in the negative control group(P=0.047).Baicalin treatment significantly down-regulated the protein expression for MMP-1(P=0.023)and MMP-9(P=0.042)and decreased the expression for MMP-2(P=0.099)compared with the negative control group.Conclusions Baicalin protects against tissue damage in ligature-induced periodontitis in rats,which might be mediated in part by its inhibitory effect on the expression of MMP-1,MMP-2 and MMP-9.
