Experimental study of Porphyromonas gingivalis on the proliferation and apoptosis of vascular endothelial cells

VernacularTitle:牙龈卟啉单胞菌影响血管内皮细胞增殖和凋亡的实验研究
Author: Dong-Mei ZHANG ¹ ; Ya-Ping PAN ; Jian ZHAO ; Li LIN ; Chen LI
Author Information

1. 中国医科大学口腔医学院
Keywords: Porphyromonas gingivalis; Atherosclerosis; Cell proliferation; Apoptosis
From: Chinese Journal of Stomatology 2008;43(6):343-346
CountryChina
Language:Chinese
Abstract: Objective To study the effects of Porphyromonas gingivalis (Pg)on the proliferation and apoptosis of vascular endothelial cell and to identify the role of Pg in the pathogenesis of atherosclerosis.Methods A cell culture model of vascular endothelial cell treated by Pg was used in vitro. The cells' ability of proliferation was measured by methyl thiazolyl tetrazolium assay, and cell cycle and the percentage of apoptosis with or without Pg invasion were examined by flow cytometry. Results Invasion of Pg could inhibit the proliferation of vascular endothelial cells at 72 h( F = 786. 68 ,P < 0. 01 ), and induce apoptosis at 24 h( F = 1074. 56 ,P < 0. 01 ). In addition, the cell cycle was arrested at G1 phase by the invasion of Pg W83 at 24 h(F=43.23,P<0.01) and ATCC 33277 at 48 h (F=66.72,P<0.01). Conclusions Pg may aggregate the inflammation of the vascular endothelial cells through induction of apoptosis , which could be one of the pathologic mechanisms in atherasclerosis.