Comparison of Mechanical Allodynia and Recovery of Locomotion and Bladder Function by Different Parameters of Low Thoracic Spinal Contusion Injury in Rats.
- Author:
Michael W CARTER
1
;
Kathia M JOHNSON
;
Jun Yeon LEE
;
Claire E HULSEBOSCH
;
Young Seob GWAK
Author Information
- Publication Type:Original Article
- Keywords: Blood brain barrier; Bladder function; Locomotion; Neuropathic pain; Spinal cord injury; Parameters; Rats
- MeSH: Animals; Blood-Brain Barrier; Contusions*; Hyperalgesia*; Locomotion*; Neuralgia; Rats*; Rats, Sprague-Dawley; Spinal Cord; Spinal Cord Injuries; Urinary Bladder*
- From:The Korean Journal of Pain 2016;29(2):86-95
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: The present study was designed to examine the functional recovery following spinal cord injury (SCI) by adjusting the parameters of impact force and dwell-time using the Infinite Horizon (IH) impactor device. METHODS: Sprague-Dawley rats (225-240 g) were divided into eight injury groups based on force of injury (Kdyn) and dwell time (seconds), indicated as Force-Dwell time: 150-4, 150-3, 150-2, 150-1, 150-0, 200-0, 90-2 and sham controls, respectively. RESULTS: After T10 SCI, higher injury force produced greater spinal cord displacement (P < 0.05) and showed a significant correlation (r = 0.813) between the displacement and the force (P < 0.05). In neuropathic pain-like behavior, the percent of paw withdrawals scores in the hindpaw for the 150-4, 150-3, 150-2, 150-1 and the 200-0 injury groups were significantly lowered compared with sham controls (P < 0.05). The recovery of locomotion had a significant within-subjects effect of time (P < 0.05) and the 150-0 group had increased recovery compared to other groups (P < 0.05). In addition, the 200-0 and the 90-2 recovered significantly better than all the 150 kdyn impact groups that included a dwell-time (P < 0.05). In recovery of spontaneous bladder function, the 150-4 injury group took significantly longer recovery time whereas the 150-0 and the 90-2 groups had the shortest recovery times. CONCLUSIONS: The present study demonstrates SCI parameters optimize development of mechanical allodynia and other pathological outcomes.
