Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis.
- Author:
Siju JOSEPH
1
;
Naicy THOMAS
;
E THANGAPANDIAN
;
Vijendra P SINGH
;
Rishendra VERMA
;
S K SRIVASTAVA
Author Information
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't ; Comparative Study ; Evaluation Studies
- Keywords: ELISA; leptospirosis; microscopic agglutination test; nLipL21; rLipL21
- MeSH: Animals; Antibodies, Bacterial/blood; Antigens, Bacterial/biosynthesis/*chemistry/genetics; Bacterial Outer Membrane Proteins/biosynthesis/*chemistry/genetics; Cattle; Cattle Diseases/blood/*microbiology; Enzyme-Linked Immunosorbent Assay/veterinary; Leptospira interrogans/*isolation & purification; Leptospirosis/blood/microbiology/*veterinary; Lipoproteins/biosynthesis/*chemistry/genetics; Recombinant Proteins/biosynthesis/chemistry/genetics; Sensitivity and Specificity
- From:Journal of Veterinary Science 2012;13(1):99-101
- CountryRepublic of Korea
- Language:English
- Abstract: A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.
