Simultaneous determination of eleven flavonoid glycosides in ginkgo biloba leaves collected in different seasons by UPLC PDA method.
- Author:
Yi-Yi ZHAO
1
;
Jing-Hui WANG
;
Xin-Tong FU
;
You-Gen CHEN
;
Hong-Zhu GUO
Author Information
1. Beijing Institute for Drug Control, Beijing 100035, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
analysis;
chemistry;
Flavonoids;
analysis;
chemistry;
Ginkgo biloba;
chemistry;
Glycosides;
analysis;
chemistry;
Molecular Structure;
Plant Leaves;
chemistry;
Plants, Medicinal;
chemistry;
Quality Control;
Reproducibility of Results;
Seasons
- From:
Acta Pharmaceutica Sinica
2013;48(1):98-103
- CountryChina
- Language:English
-
Abstract:
A new UPLC method was developed for the simultaneous determination of eleven characteristic flavonoid glycosides in Ginkgo biloba leaves. The natural occurrence of flavonoid glycosides in Ginkgo biloba leaves within one vegetative season was investigated for the first time. The analysis was performed on an Agilent ZORBAX Eclipse Plus C18 column (50 mm x 4.6 mm, 1.8 microm), the mobile phase A was acetonitrile, the mobile phase B was 0.4% phosphate aqueous solution in a gradient elution at a flow rate of 0.6 mL x min(-1), the detection was carried out at 360 nm. The result showed that eleven flavonoid glycosides had good linearity with good average recovery, separately. The method was proved to be accurate, rapid and good reproducible for the quality evaluation of Ginkgo biloba leaves, and provide an easy and rapid means for the quantitative analysis of flavonoid glycosides and their content fluctuation with seasons.
