The construction of over-expression vector for Panax notoginseng SS gene and its transformation.
- Author:
Ying SUN
1
;
Heng-Wei ZHAO
;
Feng GE
;
Lei SHI
;
Di-Qiu LIU
Author Information
1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
- Publication Type:Journal Article
- MeSH:
Agrobacterium tumefaciens;
Amino Acid Sequence;
Cell Line;
Cloning, Molecular;
DNA, Complementary;
genetics;
Farnesyl-Diphosphate Farnesyltransferase;
genetics;
metabolism;
Gene Transfer Techniques;
Genetic Vectors;
genetics;
Panax notoginseng;
chemistry;
cytology;
genetics;
microbiology;
Plants, Genetically Modified;
chemistry;
cytology;
genetics;
microbiology;
Plants, Medicinal;
chemistry;
cytology;
genetics;
microbiology;
Saponins;
metabolism;
Transformation, Genetic
- From:
Acta Pharmaceutica Sinica
2013;48(1):138-143
- CountryChina
- Language:Chinese
-
Abstract:
PNS (Panax notoginseng saponins) is the main medical bioactive component in Panax notoginseng. The medical value of PNS cannot be extended because of its low production. With the deep study of saponins biosynthetic pathway, the control of PNS biosynthesis through metabolic engineering has gradually become possible. In this study, the Squalene synthase (SS) over-expression vector was established. By the way of agrobacterium-mediated method, the vector was transfered and integrated into the Panax notoginseng genome. The result of the PCR detection and the saponin content detection shows that over-expression SS is able to produce high level of Panax notoginseng saponins, and confirms the regulatory function of SS in the biosynthesis of ginsenosides in Panax notoginseng. It provides a theoretical basis and technical basis for the construction of PNS homologous or heterologous efficient expression system in the future.
