Establishment of zebrafish osteopenia model induced by dexamethasone.
- Author:
Ying-Jie WEI
1
;
Chang-Mei WANG
;
Xue-Ting CAI
;
Yang ZHAN
;
Xiao-Bin JIA
Author Information
1. Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China. wyj970@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Density;
drug effects;
Bone Density Conservation Agents;
pharmacology;
therapeutic use;
Bone Diseases, Metabolic;
chemically induced;
prevention & control;
Calcification, Physiologic;
drug effects;
Dexamethasone;
toxicity;
Disease Models, Animal;
Etidronic Acid;
pharmacology;
therapeutic use;
Larva;
drug effects;
growth & development;
Zebrafish
- From:
Acta Pharmaceutica Sinica
2013;48(2):255-260
- CountryChina
- Language:Chinese
-
Abstract:
Zebrafish was selected as model animal, and glucocorticoid dexamethasone was used as a model compound to establish a rapid and high efficient osteopenia model. Zebrafish larvae at 4 days post fertilization (dpf) were exposed to a serial concentrations of dexamethasone solutions, and 0.5% DMSO was selected as the vehicle control group. All groups were incubated in 24-well plates (28.5 degrees C) until 9 dpf. In addition, effects of 10 micromol x L(-1) dexamethasone on preventing against osteopenia induced by etidronate disodium were also investigated. Zebrafish bones at 9 dpf were stained with alizarin red. Quantitative analysis of the stained area was performed by microscopic inspection and digital imaging methods to reflect the amount of bone mineralization. Results showed that dexamethasone group at 2.5, 10 and 25 micromol x L(-1) can decrease the staining area and the staining optical density values of zebrafish head bones when compared with the vehicle control group (0.5% DMSO), which suggested that dexamethasone can significantly reduce the zebrafish mineralized bone and the bone mineral density. Results also showed that 15 and 30 microg x mL(-1) etidronate disodium can increase the mineralized matrix of zebrafish head bone and prevent against osteopenia induced by dexamethasone. In conclusion, the study indicated that zebrafish can be an idea osteopenia model induced by dexamethasone.
