Preliminary study on pH-sensitive lipid bilayer-coated mesoporous silica nanoparticles as a novel drug carrier for antitumor drug.
- Author:
Fei-Fei LI
1
;
Xin-Xin ZHANG
;
Shi-Yan GUO
;
Yong GAN
;
Juan LI
Author Information
1. Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
administration & dosage;
pharmacology;
Camptothecin;
administration & dosage;
analogs & derivatives;
pharmacology;
Cell Proliferation;
drug effects;
Drug Carriers;
Humans;
Hydrogen-Ion Concentration;
Lipid Bilayers;
administration & dosage;
chemistry;
MCF-7 Cells;
Nanoparticles;
Particle Size;
Porosity;
Silicon Dioxide;
administration & dosage;
chemistry
- From:
Acta Pharmaceutica Sinica
2013;48(2):291-297
- CountryChina
- Language:Chinese
-
Abstract:
This study plans to prepare lipid bilayer-coated mesoporous silica nanoparticles (LMSNs) which are pH sensitive with core-shell structure to improve the tumor cell lethality of antitumor drug. The lipid coated mesoporous silica nanoparticles loaded with irinotecan (CPT-11) (CPT-11-LMSNs) were prepared by hot water-film hydration method, and the characterized its morphology, particle size and release in vitro. Meanwhile, the intracellular uptake and cell toxicity of CPT-11-LMSNs and intracellular accumulation of CPT-11 were evaluated on human breast carcinoma cell line (MCF-7). The results indicated that the mean diameter of the spherical LMSNs was (120.27 +/- 5.91) nm. The slow release in simulated normal physiological conditions and a rapid release under simulated intracellular condition demonstrated the pH sensitivity of CPT-11-MSNs in vitro. Moreover, the CPT-11-LMSN could improve the intracellular CPT-11 cumulant 2.1 times and reduce half maximal inhibitory concentration (IC50) values of CPT-11 1.4 times compared with CPT-11-MSNs, demonstrating a stronger cell lethality.
