Preparation and in vitro evaluation of pDNA-CaPi-PLGA nanoparticles with a core-shell structure.
- Author:
Jie TANG
1
;
Jing LIU
;
Ting-Ting HU
;
Cheng-Li YANG
;
Gang GUO
;
Gu HE
;
Yuan HUANG
;
Yu ZHENG
Author Information
1. West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Calcium Phosphates;
administration & dosage;
chemistry;
toxicity;
Cell Survival;
drug effects;
DNA;
administration & dosage;
chemistry;
toxicity;
Drug Carriers;
Genetic Vectors;
HEK293 Cells;
Humans;
Lactic Acid;
administration & dosage;
chemistry;
Nanoparticles;
Particle Size;
Plasmids;
genetics;
Polyglycolic Acid;
administration & dosage;
chemistry;
Transfection
- From:
Acta Pharmaceutica Sinica
2013;48(2):298-304
- CountryChina
- Language:Chinese
-
Abstract:
To develop a core-shell structure pDNA-CaPi-PLGA nanoparticles (CS-pDNA-CaPi-PLGA-NPs), calcium phosphate-pDNA nano complexes (CaPi-pDNA) were encapsulated inside of PLGA shells. The characteristics of the nanoparticles, including morphology, average particle size, zeta potential, entrapment efficiency, loading efficiency, stability in medium, pDNA protection ability from nuclease degradation, in vitro release, cytotoxicity and cell transfection were investigated and compared with the embedded structured CaPi modified PLGA nanoparticles (embedded-pDNA-CaPi-PLGA-NPs). The results showed that the obtained CS-pDNA-CaPi-PLGA-NPs were spherical in shape with an average particle size of (155 +/- 4.5) nm, zeta potentials of (-0.38 +/- 0.1) mV, entrapment efficiency of (80.56 +/- 2.5)% and loading efficiency of (1.16 +/- 0.04)%. The CS-pDNA-CaPi-PLGA-NPs were stable in the release media and could protect pDNA against nuclease degradation. And they also exhibited sustained release of pDNA in vitro. The highest gene transfection efficiency of the CS-pDNA-CaPi-PLGA-NPs in vitro reached (24.66 +/- 0.46)% (after 72 h transfection), which was significantly higher than that of free pDNA [(0.33 +/- 0.04)%, P < 0.01] and the pDNA-PLGA-NPs [(1.5 +/- 0.07)%, P < 0.01]. Besides, the transfection lasted for longer time than that of embedded-pDNA-CaPi-PLGA-NPs and the cytotoxicity of it was significantly lower than that of PEI (P < 0.01). These results indicate that CS-pDNA-CaPi-PLGA-NPs are a promising non-viral gene vector. Key words: gene delivery system; polylactic-co-glycolic acid; calcium phosphate; nanoparticle
