Effects of sequoyitol on expression of NADPH oxidase subunits p22 phox and p47 phox in rats with type 2 diabetic liver disease.
- Author:
Xiang-Pan CHEN
1
;
Jie-Ren YANG
;
Xian-Wei LI
;
Wei HAO
;
Yan LIU
;
Jun-Xiu ZHANG
Author Information
1. Department of Pharmacology, Third-Grade Pharmacology Laboratory of State Administration of Traditional Chinese Medicine, Wannan Medical College, Wuhu 241002, China.
- Publication Type:Journal Article
- MeSH:
Alanine Transaminase;
blood;
Animals;
Aspartate Aminotransferases;
blood;
Blood Glucose;
metabolism;
Diabetes Mellitus, Experimental;
chemically induced;
metabolism;
Hydrogen Peroxide;
metabolism;
Hypoglycemic Agents;
pharmacology;
Inositol;
analogs & derivatives;
pharmacology;
Insulin;
blood;
Liver;
metabolism;
pathology;
Liver Diseases;
metabolism;
Male;
NADPH Oxidases;
genetics;
metabolism;
Nitric Oxide;
metabolism;
Oxidation-Reduction;
drug effects;
Oxidative Stress;
drug effects;
RNA, Messenger;
metabolism;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Streptozocin
- From:
Acta Pharmaceutica Sinica
2013;48(4):489-494
- CountryChina
- Language:Chinese
-
Abstract:
This study is to observe the effects of sequoyitol on the expression of NADPH oxidase subunits p22 phox and p47 phox in rats with type 2 diabetic liver diseases. The model of high fat and high sugar diet as well as intraperitoneal injection of small dose of streptozotocin (STZ, 35 mg x kg(-1)) induced diabetic rat liver disease was used. After sequoyitol (50, 25 and 12.5 mg x kg(-1)) was administrated for 6 weeks, the contents of blood glucose (BG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total antioxidant capacity (T-AOC), hydrogen peroxide (H2O2), NO and insulin (Ins) were measured, liver p22 phox and p47 phox mRNA content was determined with real-time PCR and the expression of p22 phox and p47 phox protein was examined by Western blotting. In addition, pathological changes in liver were observed with HE staining. Sequoyitol could reduce the content of fasting blood glucose, ALT, AST, Ins and H2O2, restore insulin sensitive index (ISI) and weight, elevate liver tissue T-AOC and NO content, reduce the NADPH oxidase subunit liver tissue p22 phox and p47 phox mRNA and protein expression, as well as ameliorate liver pathologic lesions. The results showed that sequoyitol can ease the type 2 diabetic rat liver oxidative stress by lowering NADPH oxidase expression.
