Molecular identification of raw materials from lian qiao bai du wan.
- Author:
Zhan-Hu CUI
1
;
Chao JIANG
;
Min-Hui LI
;
Min CHEN
;
Li-She ZHOU
;
Yuan YUAN
Author Information
1. Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014060, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Chloroplasts;
genetics;
Cluster Analysis;
DNA Barcoding, Taxonomic;
DNA, Chloroplast;
genetics;
DNA, Intergenic;
genetics;
DNA, Plant;
genetics;
Drugs, Chinese Herbal;
chemistry;
Forsythia;
chemistry;
genetics;
Phylogeny;
Plants, Medicinal;
chemistry;
genetics;
Polymerase Chain Reaction;
Ribulose-Bisphosphate Carboxylase;
genetics;
Sequence Analysis, DNA;
Species Specificity
- From:
Acta Pharmaceutica Sinica
2013;48(4):590-596
- CountryChina
- Language:Chinese
-
Abstract:
Lian Qiao Bai Du Wan was used to study the identification of Chinese patent medicine by molecular marker technique. DNA was extracted through modified CTAB method. The psbA-trnH and rbcL sequences were gradient amplified, and PCR products were ligated with the pEASY-T5 vector and then transformed into Trans1-T1 cells, respectively. Clones were selected randomly and sequenced. All sequences were analyzed by BlastN and the neighbor-joining (NJ) phylogenetic tree was constructed by MEGA 4.0. The results showed that nine kinds of medicinal materials can be identified by psbA-trnH sequences, and six kinds of medicinal materials by rbcL sequences from Lian Qiao Bai Du Wan. Molecular marker technique can stably and accurately distinguish multi-origin medicinal materials in Chinese patent medicine.
