Effect of Ebosin on the inflammatory cytokines in THP-1 cells.
- Author:
Yang ZHANG
1
;
Lian-Hong GUO
;
Yuan LI
Author Information
1. Key Laboratory of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
ADAM Proteins;
metabolism;
ADAM17 Protein;
Anti-Inflammatory Agents;
pharmacology;
Caspase 1;
metabolism;
Cell Line, Tumor;
Humans;
Interleukin-1beta;
genetics;
metabolism;
Interleukin-6;
genetics;
metabolism;
Leukemia, Monocytic, Acute;
metabolism;
pathology;
Polysaccharides, Bacterial;
biosynthesis;
pharmacology;
RNA, Messenger;
metabolism;
Streptomyces;
metabolism;
Tumor Necrosis Factor-alpha;
genetics;
metabolism
- From:
Acta Pharmaceutica Sinica
2013;48(5):661-667
- CountryChina
- Language:Chinese
-
Abstract:
Ebosin is a novel exopolysaccharide produced by Streptomyces sp.139 with remarkable activity against rheumatic arthritis in vivo. In this paper, we reported effects of Ebosin on the inflammatory cytokines including interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNFalpha) in THP-1 cells. With the special fluorogenic peptide as substrates, the enzymatic activities of interleukin-1beta converting enzyme (ICE) and TNFalpha-converting enzyme (TACE) were inhibited by Ebosin separately. Using the real-time reverse transcription polymerase chain reaction (real-time PCR), the mRNA synthesis of the three cytokines were identified decline separately by Ebosin. The secretion quantum of three cytokines in THP-1 cells with Ebosin was lower than that of normal THP-1 cells determined by ELISA assay and Western blotting. All of these results showed that Ebosin has remarkably suppressed synthesis of the three cytokines in THP-1 cells through different pathways. The primary study of Ebosin on anti-inflammation mechanism was promoted developing the new drugs treating rheumatic arthritis.
