Effect of shenfu injection on CYP450s of rat liver.
- Author:
Han LI
1
;
Yu-Guang WANG
;
Zeng-Chun MA
;
Si-Si ZHOU
;
Qian-De LIANG
;
Cheng-Rong XIAO
;
Hong-Ling TAN
;
Xiang-Lin TANG
;
Hua LI
;
Guo-Lin SHEN
;
Bo-Li ZHANG
;
Yue GAO
Author Information
1. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
- Publication Type:Journal Article
- MeSH:
Aconitum;
chemistry;
Animals;
Aryl Hydrocarbon Hydroxylases;
genetics;
metabolism;
Cytochrome P-450 CYP1A2;
genetics;
metabolism;
Cytochrome P-450 CYP2B1;
genetics;
metabolism;
Cytochrome P-450 CYP3A;
genetics;
metabolism;
Cytochrome P-450 Enzyme System;
genetics;
metabolism;
Cytochrome P450 Family 2;
Drug Combinations;
Drugs, Chinese Herbal;
administration & dosage;
isolation & purification;
pharmacology;
Injections;
Male;
Microsomes, Liver;
enzymology;
Panax;
chemistry;
Plants, Medicinal;
chemistry;
RNA, Messenger;
metabolism;
Rats;
Rats, Sprague-Dawley;
Steroid 16-alpha-Hydroxylase;
genetics;
metabolism
- From:
Acta Pharmaceutica Sinica
2013;48(5):728-733
- CountryChina
- Language:Chinese
-
Abstract:
The paper is to report the study of the effect of Shenfu injection on the enzyme activity of liver CYP450 and its mRNA level of rat liver. Microsome of rat liver was prepared after intravenous administration of Shenfu injection for 7 days. The enzyme activity was quantified by Cocktail method. Meanwhile, the mRNA expression of CYP1A2, CYP2B1/2, CYP2C11 and CYP3A1 in the liver was detected by RT-PCR. Shenfu injection obviously induced the enzyme activities of CYP2B and CYP2C. Meantime Shenfu injection decreased the enzyme activities of CYP1A2 and CYP3A. The mRNA levels of CYP2B and CYP2C were also induced in rats treated with Shenfu injection. But it obviously inhibited the mRNA level of CYP1A2 and CYP3A. Since the enzyme activity and mRNA level were obviously changed after administration, the potential effect of drug-drug interaction should be concerned.
