Impact of anthracene addition on microbial community structure in soil microcosms from contaminated and uncontaminated sites.
- Author:
QingFeng WANG
1
;
ShuYing ZHANG
;
Li ZOU
;
ShuGuang XIE
Author Information
- Publication Type:Journal Article
- MeSH: Anthracenes; analysis; Bacteria; classification; genetics; isolation & purification; Biodegradation, Environmental; DNA, Bacterial; genetics; isolation & purification; Hazardous Waste; Phylogeny; Polymorphism, Restriction Fragment Length; RNA, Ribosomal, 16S; genetics; Soil Microbiology; Soil Pollutants; analysis; Waste Disposal, Fluid; methods
- From: Biomedical and Environmental Sciences 2011;24(5):543-549
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEThis paper aims to investigate the impact of anthracene addition on microbial community in agricultural soil irrigated with tap water or reclaimed wastewater.
METHODSThe changes of microbial community were characterized by terminal restriction fragment length polymorphism in combination with 16S rRNA gene clone library analysis.
RESULTSA significant change in microbial community composition was observed during the biodegradation of anthracene, with dominantly enriched members from the genus Methylophilus.
CONCLUSIONThis work might be useful for developing techniques for the isolation of novel putative PAH degrader.