The protective role of procyanidins and lycopene against mercuric chloride renal damage in rats.
- Author:
HaiBo YANG
1
;
ZhaoFa XU
;
Wei LIU
;
Yu DENG
;
Bin XU
Author Information
- Publication Type:Journal Article
- MeSH: Acetylglucosaminidase; urine; Alkaline Phosphatase; urine; Animals; Antioxidants; therapeutic use; Blood Urea Nitrogen; Carotenoids; therapeutic use; Glutathione; metabolism; Glutathione Peroxidase; metabolism; Kidney; drug effects; metabolism; pathology; Kidney Diseases; chemically induced; metabolism; pathology; prevention & control; L-Lactate Dehydrogenase; urine; Lipid Peroxidation; Malondialdehyde; metabolism; Mercuric Chloride; pharmacokinetics; toxicity; urine; Mercury; metabolism; Proanthocyanidins; therapeutic use; Rats; Rats, Wistar; Reactive Oxygen Species; metabolism; Superoxide Dismutase; metabolism
- From: Biomedical and Environmental Sciences 2011;24(5):550-559
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEThis study aims to investigate the protection of procyanidins and lycopene from the renal damage induced by mercuric chloride.
METHODSRats were treated with either procyanidins or lycopene 2h before HgCl(2) subcutaneously injection, once daily treatment for 2 successive days.
RESULTSIn comparison with HgCl(2) group, markers of renal function such as blood urea nitrogen in serum and urinary protein were decreased to (18.45±11.63) mmol/L and (15.93±9.36) mmol/L, (4.54±0.78) g/(g·Cr) and (4.40±1.12) g/(g·Cr). N-acetyl-beta-D-glucosaminidase, lactate dehydrogenase, alkaline phosphatase in urine were depressed to (125.49±11.68) U/(g·Cr), (103.73±21.79) U/(g·Cr), (101.99±12.28) U/(g·Cr), and (113.19±23.74) U/(g·Cr), (71.14±21.80) U/(g·Cr), (73.64±21.51) U/(g·Cr) in procyanidins and lycopene groups. Indicators of oxidative stress, for example, Glutathion was reduced to (45.58±9.89) μmol/(g·pro) and (45.33±5.90) μmol/(g·pro), and antioxidant enzymes such as superoxide dismutase, glutathione-peroxidase were enhanced to (43.07±10.97) U/(mg·pro) and (39.94±6.04) U/(mg·pro), (83.85±18.48) U/(mg·pro), and (85.62±12.68) U/(mg·pro). Malondialdehyde was lowered to (0.95±0.12) (μmol/g·pro) and (1.03±0.12) μmol/(g·pro) in procyanidins and lycopene groups. ROS generation was decreased by 27.63% and 16.40% and apoptosis was also decreased in procyanidins and lycopene groups respectively. Pathological changes were much better as well.
CONCLUSIONProcyanidins and Lycopene play some protective role against mercury kidney damage.
