Gene expression changes in the pituitary gland of rats exposed to electromagnetic pulses.
- Author:
YuHong QI
1
;
Jun LIANG
;
YanPing HUI
;
GuiRong DING
;
JunYe LIU
;
XiaoMing SU
;
GuoZhen GUO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoma; genetics; metabolism; pathology; Animals; Down-Regulation; Electromagnetic Phenomena; Female; Gene Expression Profiling; Oligonucleotide Array Sequence Analysis; PTEN Phosphohydrolase; genetics; metabolism; Pituitary Gland; metabolism; Pituitary Neoplasms; genetics; metabolism; pathology; Proto-Oncogene Proteins c-jun; genetics; metabolism; Rats; Rats, Wistar; Real-Time Polymerase Chain Reaction
- From: Biomedical and Environmental Sciences 2011;24(5):560-568
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEWe examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).
METHODSThe global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis. We then validated and further investigated the reduced expression of two tumorigenesis-related genes, Pten, and Jund, by assessing their mRNA and protein expression by quantitative real-time-PCR, western blotting, and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.
RESULTSEMP exposure induced genome-wide gene expression changes in the rat pituitary gland. There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.
CONCLUSIONEMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland. These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.
