Establishment of hamster- and human-PRNP transgenic mice.
- Author:
Han Shi GONG
1
;
Chan TIAN
;
Bao Yun ZHANG
;
Zhao Yun WANG
;
Wu Ling XIE
;
Yuan Yuan JING
;
Chen GAO
;
Hui Ying JIANG
;
Qi SHI
;
Yong LIU
;
Xiao Ping DONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Cricetinae; DNA; genetics; Disease Models, Animal; Humans; Immunohistochemistry; Mice; Mice, Inbred C57BL; Mice, Transgenic; Organ Specificity; Plasmids; Prion Diseases; genetics; Prion Proteins; Prions; genetics; Real-Time Polymerase Chain Reaction; Transcription, Genetic
- From: Biomedical and Environmental Sciences 2011;24(6):608-616
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo create transgenic mice expressing hamster- and human-PRNP as a model for understanding the physiological function and pathology of prion protein (PrP), as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies (TSEs).
METHODSHamster and human-PRNP transgenic mice were established by conventional methods. The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR. PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR, real-time RT-PCR, and western blot analysis. Histological analyses of transgenic mice were performed by hematoxylin and eosin (H & E) staining and immunohistochemical (IHC) methods.
RESULTSIntegrated PRNP copy number in various mouse lines was 53 (Tg-haPrP1), 18 (Tg-huPrP1), 3 (Tg-huPrP2), and 16 (Tg-huPrP5), respectively. Exogenous PrPs were expressed at both the transcriptional and translational level. Histological assays did not detect any abnormalities in brain or other organs.
CONCLUSIONWe have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines. These four transgenic mouse lines provide ideal models for additional research.
