ZnO, TiO(2), SiO(2,) and Al(2)O(3) nanoparticles-induced toxic effects on human fetal lung fibroblasts.
- Author:
Xiao Qiang ZHANG
1
;
Li Hong YIN
;
Meng TANG
;
Yue Pu PU
Author Information
- Publication Type:Journal Article
- MeSH: Aluminum Oxide; chemistry; toxicity; Apoptosis; drug effects; Cell Culture Techniques; Cell Line; Cell Shape; drug effects; Cell Survival; drug effects; Dose-Response Relationship, Drug; Fibroblasts; drug effects; pathology; Humans; Lung; drug effects; embryology; pathology; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Microscopy, Phase-Contrast; Nanoparticles; chemistry; toxicity; Silicon Dioxide; chemistry; toxicity; Surface Properties; Titanium; chemistry; toxicity; Zinc Oxide; toxicity
- From: Biomedical and Environmental Sciences 2011;24(6):661-669
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEThis study aims to investigate and compare the toxic effects of four types of metal oxide (ZnO, TiO(2), SiO(2,) and Al(2)O(3)) nanoparticles with similar primary size (∼20 nm) on human fetal lung fibroblasts (HFL1) in vitro.
METHODSThe HFL1 cells were exposed to the nanoparticles, and toxic effects were analyzed by using MTT assay, cellular morphology observation and Hoechst 33 258 staining.
RESULTSThe results show that the four types of metal oxide nanoparticles lead to cellular mitochondrial dysfunction, morphological modifications and apoptosis at the concentration range of 0.25-1.50 mg/mL and the toxic effects are obviously displayed in dose-dependent manner. ZnO is the most toxic nanomaterials followed by TiO(2), SiO(2), and Al(2)O(3) nanoparticles in a descending order.
CONCLUSIONThe results highlight the differential cytotoxicity associated with exposure to ZnO, TiO(2), SiO(2), and Al(2)O(3) nanoparticles, and suggest an extreme attention to safety utilization of these nanomaterials.
