Evaluation of a new real-time PCR assay for detection of Mycoplasma pneumoniae in clinical specimens.

Author: Fei ZHAO ¹ ; Bin CAO ; Li Hua HE ; Yu Dong YIN ; Xiao Xia TAO ; Shu Fan SONG ; Fan Liang MENG ; Jian Zhong ZHANG
Author Information

1. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Publication Type:Journal Article
MeSH: Genes, Bacterial; Humans; Mycoplasma pneumoniae; genetics; isolation & purification; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity; Sequence Analysis, DNA
From: Biomedical and Environmental Sciences 2012;25(1):77-81
CountryChina
Language:English
Abstract:
OBJECTIVETo establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.
METHODSBy analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China, an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed. The specificity and sensitivity of this assay were evaluated and compared with other two reported assays (RepMp1 and Mp181) using 40 positive and 100 negative clinical specimens.
RESULTSThe detection limit of the new assay was 8.1 fg (about 1∼3CFU) M.pneumoniae DNA. The sensitivity of MpP1, RepMp1, and Mp181 assays appeared to be 100%, 100%, and 85%, respectively.
CONCLUSIONMpP1 assay is suitable for the detection of M.pneumoniae in Chinese clinical specimens.