Comparison of lipopolysaccharide and protein immunogens from pathogenic Yersinia enterocolitica bio-serotype 1B/O:8 and 2/O:9 using SDS-PAGE.
- Author:
Wen Peng GU
1
;
Xin WANG
;
Hai Yan QIU
;
Xia LUO
;
Yu Chun XIAO
;
Liu Ying TANG
;
Biao KAN
;
Jian Guo XU
;
Huai Qi JING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Bacterial; genetics; metabolism; Bacterial Proteins; genetics; metabolism; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Female; Gene Expression Regulation, Bacterial; physiology; Lipopolysaccharides; metabolism; Rabbits; Yersinia enterocolitica; classification; metabolism
- From: Biomedical and Environmental Sciences 2012;25(3):282-290
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEYersinia enterocolitica is an extracellular pathogen and its related antigens interact with the host immune system. We investigated the difference in immunological characteristics between a highly pathogenic and poorly pathogenic strain of Y. enterocolitica.
METHODSWe used SDS-PAGE and western blotting to characterize lipopolysaccharide (LPS), Yersinia outer membrane proteins (Yops), membrane proteins, and whole-cell proteins from poorly pathogenic Y. enterocolitica bio-serotype 2/O:9, isolated from China, and highly pathogenic bio-serotype 1B/O:8, isolated from Japan.
RESULTSThese two strains of Y. enterocolitica had different LPS immune response patterns. Comparison of their Yops also showed differences that could have accounted for their differences in pathogenicity. The membrane and whole-cell proteins of both strains were similar; immunoblottting showed that the 35 kD and perhaps the 10 kD proteins were immunogens in both strains.
CONCLUSIONThe major antigens of the two strains eliciting the host immune response were the LPS and membrane proteins, as shown by comparing protein samples with reference and purified preparations.
