Inhibitory effect of extracellular polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to cornea in vitro.
- Author:
Hao CHEN
1
;
Zhou ZHENG
;
Peng CHEN
;
Xiang Gen WU
;
Ge ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Candida; drug effects; physiology; Cell Adhesion; drug effects; Cornea; microbiology; Humans; Phosphorylation; Polysaccharides, Bacterial; pharmacology; Proto-Oncogene Proteins c-akt; metabolism; Pseudoalteromonas; metabolism
- From: Biomedical and Environmental Sciences 2012;25(2):210-215
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEFungal keratitis (FK) is a vision-threatening infection, whose treatment requires more effective and safer anti-fungal agent exploitation urgently. With this aim, we focused on the effect of an extracellular polysaccharide on fungal adhesion to human corneal epithelial cells.
METHODSWe performed the cytotoxicity assays of the extracellular polysaccharide EPS-II from an antarctic bacterium Pseudoaltermonas and evaluated its inhibitory effect on Candida albicans cells' adherence to human corneal epithelial cells (HCECs).
RESULTSEPS-II, which displayed minor cytotoxicity but also promoted proliferation of HCECs, could inhibit the adherence of yeast cells to HCECs in a dose-dependent manner. EPS-II could also suppress the subsequent PI3K/AKT signaling pathway, and thereby decrease the expression of early inflammatory cytokines.
CONCLUSIONSExtracellular polysaccharide EPS-II was suggested as a new natural agent for attenuating FK.
