Effects of endothelial progenitor cell-conditioned medium on the proliferation, adhesion and migration of vascular smooth muscle cells.
- Author:
Hai-Yan WU
1
;
Hai-Rong CHU
;
Hong LI
;
Ke-Xin TANG
;
Xu JING
;
Jin-Long SUN
;
Qing-Ling YIN
;
Min CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Adhesion; Cell Movement; Cell Proliferation; Cells, Cultured; Culture Media, Conditioned; chemistry; Endothelial Cells; cytology; Male; Muscle, Smooth, Vascular; cytology; Rats; Rats, Sprague-Dawley; Stem Cells; cytology
- From: Chinese Journal of Applied Physiology 2013;29(3):199-202
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of endothelia progenitor cells conditioned medium (EPC-CM) on the migration, adhesion and proliferation of vascular smooth muscle cells (VSMCs).
METHODSMononuclear cells were isolated from rat bone marrow by density gradient centrifugation,plated on dishes precoated with 5% fibronectin, and then cultured with complete M199 medium (including 15% fetal calf serum, 10 microg/L VEGF and 5 microg/L bFGF). EPC-CM was collected and used to incubate VSMCs isolated from rat arteriae aorta. After 24 h, VSMCs proliferation, adhesion and migration were assayed with CCK-8, adhesion test and modified Boyden chamber assay, respectively.
RESULTSThe proliferation, adhesion and migration of VSMCs were obviously decreased when the cells were cultured with EPC-CM.
CONCLUSIONEPC-CM could inhibit VSMC functions, which would be one of the mechanisms against atherosclerosis by EPCs.
