Effect of hypoxia on gastric cancer cell proliferation and LINE-1 endonuclease variant GCRG213 expression.
- Author:
Yu TIAN
1
;
Xin HUANG
;
Tong ZHAO
;
Ling-Ling ZHU
;
Gang-Shi WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; Cells, Cultured; Deoxyribonuclease I; genetics; Gastric Mucosa; cytology; Gene Expression; Humans; Hypoxia; Peptide Hormones; genetics; Stomach Neoplasms; genetics; pathology
- From: Chinese Journal of Applied Physiology 2013;29(3):225-228
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the change of gastric cancer cell proliferation and the expression of gastric cancer related gene 213 (GCRG213), a long interspersed nuclear element-1 (LINE-1) endonuclease variant, during hypoxia.
METHODSNormal gastric mucosa cell GES-1 and gastric cancer cell BGC-823 were cultured in 20% or 3% oxygen concentrations, respectively. MTT test was used to analyze the proliferation of the GES-1 and BGC-823 cells. The change of GCRG213 mRNA and protein expression in GES-1 and BGC-823 cells was detected by using RT-PCR and Western blot analysis. Blast was used at the NCBI Blast server to identify GCRG213 sequence to any alignment in the GeneBank databases.
RESULTSCompared with 20% oxygen condition, 3% oxygen concentration could promote cell growth. Mean-while, the expression of GCRG213 at mRNA and protein levels was increased. GCRG213 sequence shared high homology with LINE-1 endonuclease sequence.
CONCLUSIONGCRG213 is a variant of LINE-1 endonuclease. Hypoxia as in 3% oxygen condition can promote cell proliferation and lead to GCRG213 overexpression.
