Choline promotes angiogenesis in chick embryo chorioallantoic membrane.
- Author:
Hui WANG
1
;
Xiang-Zhi ZENG
;
Wen-Yu CUI
;
Lian DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chick Embryo; blood supply; drug effects; Choline; pharmacology; Chorioallantoic Membrane; blood supply; drug effects; Neovascularization, Physiologic; drug effects
- From: Chinese Journal of Applied Physiology 2013;29(3):229-231
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate choline promoting angiogenesis on chick embryo chorioallantoic membrane (CAM).
METHODSCAM model was prepared, the choline chloride, human vascular endothelial growth factor (hVEGF) and normal saline were added respectively onto the carrier on the CAM, the state of angiogenesis was observed and the number of new blood vessels was counted.
RESULTSCholine chloride was tested at the concentrations of 0.5 nmol/L - 1 mmol/L in this experiment, when its concentrations were increased to 0.01 micromol/L - 1 000 micromol/L, it could stimulate angiogenesis, the minimum effective concentration was tested as 0.01 micromol/L, and its effect for promoting the angiogenesis was equivalent to that of hVEGF, the potent stimulator for angiogenesis.
CONCLUSIONThe result shows that choline can promote angiogenesis in the chick embryo chorioallantoic membrane.