The effect of PPARalpha signal channel on atorvastatin inhibiting MMP-9 expression in aging myocytes.
- Author:
Lei HAN
1
;
Ping YE
2
;
Ming-Gao LI
3
Author Information
- Publication Type:Journal Article
- MeSH: Aging; Animals; Atorvastatin Calcium; Cells, Cultured; Heptanoic Acids; pharmacology; Matrix Metalloproteinase 9; metabolism; Muscle Cells; drug effects; metabolism; Oxazoles; pharmacology; PPAR alpha; metabolism; Pyrroles; pharmacology; Rats; Rats, Wistar; Signal Transduction; Tyrosine; analogs & derivatives; pharmacology
- From: Chinese Journal of Applied Physiology 2013;29(5):469-472
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the correlation between atorvastatin inhibiting the expression level of matrix metalloproteinase 9 (MMP-9) and peroxisome proliferator activated receptor alpha (PPARalpha) signal channel in myocyte of aging rat.
METHODSPrimary cultures of myocyte were got ten from aging rats. Myocyte were divided into control group, DMSO group, atorvastatin group, atorvastatin plus GW6471 group, which treated respectively by cell culture medium, DMSO, atorvastatin, atorvastatin plus GW6471. The expression of MMP-9 mRNA was evaluated by RT-PCR, and content of MMP-9 protein was detected by Western blot.
RESULTS(1) There was no difference between control group and DMSO group in level of MMP-9 mRNA and protein expression (P > 0.05); (2) The level of MMP-9 mRNA and MMP-9 protein expression in atorvastatin group were significantly lower than those in control group (P < 0.01); (3) Both level of MMP-9 mRNA and protein expression in atorvastatin plus GW6471 group were significantly higher than those in atorvastatin group (P < 0.05), but were still lower than those in control group (P < 0.05).
CONCLUSIONAtorvastatin inhibit MMP-9 expression of aging myocytes by PPARalpha signal channel.
