Analysis on genetic stability in different development stages of Dendrobium huoshanense by RAPD.
- Author:
Shi-Quan LIU
1
;
Xiao-Jun LI
;
Qing-Bo YU
;
Hong XIE
;
Gen-Yu ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Analysis of Variance; DNA Primers; DNA, Plant; analysis; genetics; Dendrobium; genetics; growth & development; Genetic Variation; Germination; genetics; Plants, Medicinal; genetics; growth & development; Random Amplified Polymorphic DNA Technique; Time Factors; Tissue Culture Techniques
- From: China Journal of Chinese Materia Medica 2007;32(10):902-905
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo research the hereditary stability of Dendrobium huoshanense which were subcultured 7-8 times in the same tissue culture system.
METHODUsing three primers of arbitrary decamer oligonucleotide sequences from 20 primers for DNA amplification, random amplified polymorphic DNA (RAPD) were performed.
RESULTThe genetic similarity coefficient was varied from 85.4% to 98.4%. The variation of protocorm, germination and monoleaf were rather more notable than that of bileaf and inflorescence.
CONCLUSIONThe variation of the same pod is quite small. It is feasible to set up the rapid propagation system of D. huoshanense
