Determination of benzopyrene in red clover extract by high performance liquid chromatography with spectrofluorescence detector.
- Author:
Fu-Hua YAN
1
;
Bo CHEN
;
Jian-Guo ZENG
;
Jian-Rong MIAO
Author Information
- Publication Type:Journal Article
- MeSH: Benzo(a)pyrene; analysis; isolation & purification; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Fluorescence; Plants, Medicinal; chemistry; Reproducibility of Results; Trifolium; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(10):935-937
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a high performance liquid chromatography method with spectrofluorescence detector for the quantitative analysis of benzopyrene in red clover extract.
METHODAnalysis was performed on Hypersil C18 column (4. 6 mm x 200 mm, 5 microm) with acetonitrile and water (70: 30) as mobile phase; The excitation wavelength and emission wavelengh were 368 nm and 405 nm, respectively; Flow rate was 1.0 mL x min(-1).
RESULTThe linear range was 0.93-37.2 ng x mL(-1), and r = 0.9992 (n = 5). The average recovery of benzopyrene was 85.2% (n = 9).
CONCLUSIONThe method was convenient and accurate with good recovery.
