A new method to obtain molecular marker of sequence-tagged site (STS) of Panax ginseng and P. quinquefolius.
- Author:
Guang-hong CUI
1
;
Lu-qi HUANG
;
Xiao-jing TANG
;
Xi-rong HE
;
Xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; DNA Primers; DNA, Plant; genetics; DNA, Ribosomal; genetics; Genetic Markers; genetics; Molecular Sequence Data; Panax; classification; genetics; Plant Roots; genetics; Plants, Medicinal; genetics; Polymorphism, Genetic; Random Amplified Polymorphic DNA Technique; methods; Sequence Analysis, DNA; Sequence Tagged Sites; Species Specificity
- From: China Journal of Chinese Materia Medica 2007;32(11):1012-1015
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESearching a new molecular method to authenticate Panax ginseng and P. quenquefolium.
METHODSingle primers based on rDNA sequences of Panax species were designed to obtain polymorphic bands of P. ginseng and P. quinquefolius and then sequenced. Four PCR primers (two forword and two reverse primers) specific to P. ginseng and P. quinquefolius were designed.
RESULTPrimer Pg-6F, Pg-479R only amplified 474 bp band for P. ginseng and primer Pq-442F, Pq-658R only amplified 217 bp band for P. quinquefolius. It is indicated that the four primers could serve as specific STS primers for Panax species.
CONCLUSIONA new way to obtain STS primers of Panax species was established. This method is more quick and efficient than SCAR-PCR method and can serve as a model to obtain molecular markers for other Chinese material medica.
