Application of macroporous resin in purification for effective part from Polygonum cuspidatum.
- Author:
Dan LIU
1
;
Hai-feng TANG
;
San-qi ZHANG
;
Yu DING
;
Chun-e YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adsorption; Anthraquinones; chemistry; isolation & purification; Drugs, Chinese Herbal; chemistry; isolation & purification; Emodin; analogs & derivatives; isolation & purification; Fallopia japonica; chemistry; Glucosides; isolation & purification; Plants, Medicinal; chemistry; Resins, Synthetic; chemistry; Stilbenes; isolation & purification; Technology, Pharmaceutical; methods; Temperature
- From: China Journal of Chinese Materia Medica 2007;32(11):1019-1024
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the technological parameters of the purification process for effective part from Polygonum cuspidatum.
METHODUsing adsorption capacities and desorption rates of polydatin, resveratrol,emodin,physcion and total anthraquinone as the primary screening indexes, six resins were surveyed,and the optimized conditions of adsorption and desorption of the effective ingredients were studied.
RESULTResin D101 gave good separation performance and was selected to purify the effective part in Polygonum cuspidatum. The optimum parameters were established as the following: 1 BV (bed volume) sample extract was passed through the column with a flow rate of 2.4 BV x h(-1), 30 min later,the column was washed with 2 BV water, 2 BV 20% ethanol, 5 BV 50% ethanol, 2 BV 70% ethanol and 5 BV 95% ethanol, respectively. The combined 50% and 95% ethanolic elutes were concentrated to yield the purified effctive part.
CONCLUSIONThe purity of the total effective ingredients in the product was up to 36. 87%. Macroporous resin D101 could be well used in separating and purifying the effective part from Polygonum cuspidatum.
