Study on mechanism of abnormal savda munziq flavonoids in induction of apoptosis of Hep2 cells.
- Author:
Abdiryim YUSUP
1
;
Halmurat UPUR
;
Xirali TURSUN
;
Bénédicte BERKE
;
Isabelle BAUDRIMONT
;
Nicholas MOORE
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; isolation & purification; pharmacology; Apoptosis; drug effects; Carcinoma, Hepatocellular; genetics; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Drug Combinations; Flavonoids; isolation & purification; pharmacology; Flow Cytometry; Humans; Liver Neoplasms; genetics; metabolism; pathology; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-bcl-2; biosynthesis; genetics; Proto-Oncogene Proteins p21(ras); biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Tumor Suppressor Protein p53; biosynthesis; genetics; bcl-2-Associated X Protein; biosynthesis; genetics
- From: China Journal of Chinese Materia Medica 2007;32(11):1068-1071
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Abnormal Savda Munziq (ASMq) flavonoids on proliferation, apoptosis and apoptosis-related gene expression in human hepatoma (HepG2) cells in vitro and to probe the mechanism.
METHODThe effects of ASMq flavonoids on proliferation, apoptosis and apoptosis-related gene expression of HepG2 cells were investigated respectively by MTT assay, gel electrophoresis, flow cytometry and RT-PCR.
RESULTASMq flavonoids significantly inhibited growth of HepG2 cells in vitro, arrested HepG2 in the sub-G, phase, induced cell apoptosis and significantly down-regulated expression level of Bcl-2 mRNA, and up-regulated expression of p53, p21, Bax gene mRNA expressions.
CONCLUSIONASMq flavonoids has significantly regulative action on growth, apoptosis and apoptosis-related gene expression of cancer cells in vitro, which possibly are the important way to excert anticancer effect, and flavonoids are possibly a main active component of ASMq for exerting the anticancer effect.
