Effect of concentration of catalpol and 5-hydroxymethyl-2-furaldehyde from processing of Rehmanniae Radix.
- Author:
Mei-fen ZHU
1
;
Xiang-qian LIU
;
Oh JU-HEE
;
Yook CHANG-SOO
;
Lee JE-HYUN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Ethanol; Furaldehyde; analogs & derivatives; analysis; Glucosides; analysis; Hot Temperature; Iridoid Glucosides; Iridoids; analysis; Plant Tubers; chemistry; Plants, Medicinal; chemistry; Rehmannia; chemistry; Technology, Pharmaceutical; methods
- From: China Journal of Chinese Materia Medica 2007;32(12):1155-1157
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study on effect of concentration of catalpol and 5-hydroxy methyl-2-furaldehyde (5-HMF) from Rehmanniae Radix at various processing.
METHODThe Rehmanniae Radix was dried and prepared from the steaming process with 10% ethanol, 50% ethanol at 90 degrees C and 100 degrees C each other. And the changes of catalpol and 5-HMF was determinated. The extraction of 5-HMF and catalpol was sonicated in 30% methanol for 2 h. The analysis of 5-HMF and catalpol was conducted by HPLC with reversed-phase C-18 column and detected under UV 284 nm, 204 nm. Elution was carried out at 1.0 mL min(-1) with 3% acetonitrile.
RESULTFrom this analysis, we found out that the content of catalpol was decreased with the number of processing times, and content of 5-HMF was increased with the number of processing times at various processing. The temperature and concentration of ethanol can effect on content of catalpol and 5-HMF at processing. The Cooked Rehmanniae Radix processed at 100 degrees C, 10% ethanol is best. And the content of 5-HMF processed for more than 7 times was accorded with standard of Korea phamcopoetia.
CONCLUSIONAnalyze the effect of concentration of catalpol and 5-HMF from Rehmanniae Radix at various processing, and provide the foundation for further study.
